Composite

Part:BBa_K5371102:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


SIR2-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 823
    Illegal BamHI site found at 261
    Illegal BamHI site found at 809
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2038
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2038
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For continuous and complete expression of Sir2-linker-mcherry, stop codon of Sir2 is deleted to prevent a premature stop in this case. Length of the linker is adjusted to avoid frameshift, which may cause faulty translation. Codons of the linker is designed to avoid polarized amino acids or those with large residues.


Source

The Sir2 comes from genome of Saccharomyces cerevisiae. The mCherry was amplified from the plasmid pTGL0126-LV007 vector-PM（Lyn）-mCherry, kindly provided by Prof Chew Tingang.

References